PHLEBOTOMY SERVICES
EVACUATED BLOOD COLLECTION TUBE GUIDE.
Listed in Order of Draw for Blood Collection Tubes and Syringe Collections.
| STOPPER COLOR |
SYMBOL |
CONTENTS |
VOL. |
USES/COMMENTS |
|
Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. .
|
| Royal Blue |
 |
No additive (serum); special glass and stopper material |
7.0 mL |
ONLY if being used to collect trace metals (e.g. copper)* |
| Red |
 |
No additive |
7.0 mL |
Cryoglobulins and CH50 |
| Light Blue |
 |
3.2% Sodium Citrate |
4.5 mL |
PT, PTT, TCT, CMV buffy coat, Factor Activity
Tube MUST be filled 100% - No exceptions! |
| Royal Blue |
|
(K2)EDTA |
7.0 mL |
Most drug levels, and toxicology screens |
| Gold Top (Serum Separator, "SST") |
 |
Contains separating gel and clot activator |
6.0 mL |
Most chemistry, endocrine and serology tests, including Hepatitis and HIV |
Light Blue -
Yellow Label on Tube |
 |
Thrombin |
2.0 mL |
For FDP test ONLY; Obtain tube from Core Lab Coag; Allow to Clot |
| Mint Green |
 |
Lithium Heparin with PST gel (plasma separator tube) |
7.0 mL |
Most Chemistry tests |
| Dark Green with Stripes on label |
 |
Sodium heparin (100 USP Units) |
5.0 mL |
Cytogenetics, HLA Typing |
|
Dark Green with no stripes on label
|
 |
Lithium Heparin only (no gel) |
4.0 mL |
Ammonia, lactate, ionized calcium, Busulfan |
| Tan |
 |
(K2)EDTA
|
5.0 mL |
Lead levels |
| Yellow |
 |
ACD Solution A consists of trisodium citrate, citric acid and dextrose |
8.5 mL |
DNA Studies, HIV Cultures |
| Pink |
 |
(K2)EDTA |
Draws 6 mL - Minimum 4 mL |
Blood type & Screen, Compatibility Study, Direct Coombs
|
| Pearl Top (Plasma Preparation, "PPT") |
 |
Separating gel and (K2)EDTA |
4.0 mL |
Adenovirus PCR
Toxoplasma PCR
HHV-6 PCR
HIV Viral Load PCR
|
| Lavender ("Purple") |
 |
(K2)EDTA |
3.0 mL |
CBC/Diff/Retic/Sed Rate, FK506, Cyclosporin, Platelet Ab, Coombs, Flow Cytometry |
NOTE: All tubes sterile.
Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.
Blood Culture Options (Specify Culture Type when Ordering)
| Culture Type |
Patient Population |
Test ID |
Test # |
BottleType/Comments |
|
| Culture, Blood, Routine |
All Patients not Meeting Criteria Below
NOTE: This culture will recover
HACEK organisms with no
change in collection
or incubation conditions.
If Brucella or Francisella are
in the differential, extended
incubation may be required.
Please contact the
Microbiology Lab (966-4056).
|
BROU |
9500 |
BD BACTEC Plus Aerobic/F
(Grey Cap/Blue Ring)
(Optimal Volume 8-10 mL)
Lawson# 052346(UOM box of 50 bottles)
BD BACTEC Anaerobic Lytic/10
(Purple Cap/Maroon Ring)
(Optimal Volume 8-10 mL)
Lawson# 052347
(UOM box of 50 bottles)
|
|
| Culture, Blood, AFB |
HIV+ patients with CD4 Count <100;
Suspected MAC infections |
AFBB |
9684 |
BD BACTEC Myco/F Lytic
(White Cap/Red Ring)
Available from Phlebotomy Lab
(Optimal Volume 1-5 mL)
|
|
| Culture, Blood, Pediatric |
Patients Weighing <40 kg |
BPED |
9548 |
BD BACTEC Plus aerobic/F
(Grey Cap/Blue Ring)
(Optimal Bolume 8-10 mL)
For Patients <20 Kg, 1-4 mL is acceptable
For Patients 20-40 Kg, 4-8 mL in one bottle.
For Patients >40 Kg (See Routine above)
|
|
| Blood Culture Mould |
Suspected infections due to
dimorphic (endemic) fungi,
Fusarium sp., Trichosporon sp.,
Malassezia furfur (Specify at Collection).
|
BPATH |
9507 |
Isolator Yellow Top
(10 mL Volume)
Available from Plebotomy
BD BACTEC Plus Aerobic/F
(Grey Cap/Blue Ring)
(Optimal Volume 8-10 mL)
Lawson# 052346
(UOM box of 50 bottles)
BD BACTEC Myco/F Lytic
(White Cap/Red Ring)
Available from Phlebotomy Lab
(Optimal Volume 1-5 mL)
|
|
